Laboratory of microbial chromatography
Studies of microorganisms on fatty acid markers.
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Since 2010 Laboratory of microbial chromatography has been doing laboratory testing through the “Assessment of human microecology by chromatography-mass spectrometry” method by the Doctor of Biosciences Sciences Georgy Osipov.
Today, we are an independent medical laboratory that performs human microecology tests using the HMS method, both for the entire territory of Russia, and for Ukraine, Kazakhstan, Belarus, the European Union, and the United Kingdom. We operate in full compliance with the method and do not conduct rapid testing, which is often offered by some laboratories in order to shorten the time. Our laboratory conducts microecology research and has a patent for defining reference ranges of norms for final research protocols. Our equipment is regularly checked by certified service engineers trained in the USA directly by the manufacturer.
Studying biomaterials with us, you are guaranteed to have high quality results.
Essence of the test method
The test method relates to a new direction in microbiological research – diagnosis of infections, dysbiosis and inflammatory processes by specific microbial chemicals (markers). These substances are contained in the microorganisms cell walls or produced by them during their vital activity.
The diagnosis by chemical markers of microbial cells is possible because their chemical structure differs from the human cells substance. Here we deal with various fatty acids: humans have slightly over 20 species, while microbes – over 200.
Therefore, with the analysis method that is sensitive enough, it is easy to detect the microbes in the human body. The chromatography-mass spectrometry is exactly this kind of method (read more: Wikipedia — Gas chromatography–mass spectrometry).
- GC-MS is a combination of gas chromatography, a separation technique, and mass spectrometry, which is used to identify analytes. This detection technique has extensive applications in biomedicine, including disease diagnosis, pharmaceutical quality control, and metabolic profiling.
- GC-MS can be used for the bioanalysis of body fluids to detect narcotics, barbiturates, alcohols, and drugs. It is also useful in detecting pollutants and metabolites in serum and in fatty acid profiling in microbes.
- GC-MS has specific advantages that led to labeling this technology as the “gold standard” in metabolomics (Lu et al, 2008). This is due to the robust, reproducible and selective nature of the technique, as well as the large number of well-established libraries and metabolite databases available (NIST Mass Spectral Library; MIDI Sherlock).
Being supported by cutting-edge software and analysis methodologies, these methods make it possible to quickly and reliably determine small proportions of substances of microbial origin in any human biological fluids and in environmental objects. Within a few hours, it is possible to qualitatively and quantitatively determine the composition of any microorganisms, if they have markers or differ in the chemical component profile, just the way people differ in fingerprints.
Learn the details of tests by the Microbial markers chromato-mass-spectrometry method from experts.
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Chromatography vs. Genetic-based methods
Over the years, we see more and more medical practitioners taking advantage of Chromatography in their patient condition evaluation process. GC-MS is considered to be a solid alternative to genetic-based test methods, which are often outside of consideration due to economic reasons. The method of microorganisms detection by fatty acid – markers — is similar to genetic analysis (PCR, 16s rRNA nucleotide sequence determination, etc.), since the composition of fatty acids is determined in DNA and reproduced by replicating a genome portion by transport RNA and subsequent FA synthesis in mitochondria by matrix RNA.
Therefore, the bacteria fatty acid profile is their unique profile ID, similar to people’s fingerprints. It is as conservative as the DNA structure, but also susceptible to mutations under the influence of environmental factors. The stability of the fatty acids set making up the microbes cells is confirmed by studies in bacterial paleontology, which show that since 2.5 billion years ago, the composition of individual microbes fatty acids and the pool of their fatty acids as a whole have remained constant.
Cases of GC-MS method application
By today, the GC-MS method has proven itself among specialists in preventive and personalized medicine, gynecologists, reproductologists, urologists, dermatologists, gastroenterologists, space biology and medicine specialists, both as an alternative and when combined with traditional examination methods.
MedBazis Laboratory has been on the market since 2010. It is fitted with equipment manufactured by Agilent Technologies, Inc. and uses the data of the mass spectral library NIST 14 of the the National Institute of Standards and Technology (NIST), as well as the Sherlock ™ Microbial Identification System (MIS) — MIDI Sherlock.
Scientific justification and key contributors
This diagnostic method is based on numerous studies of recent decades in the field of fatty acid composition of microbial cells and chemo differentiation of microorganisms and is based on the works of prominent scientists:
G.A. OSIPOV. Bakulev Scientific center for cardiovascularsurgery, Moscow, Russia — read more
D.C.WHITE. University of Tennessee, Knoxville, USA — read more
LENNART LARSSON. Division of Medical Microbiology, Lund University, Lund, Sweden — read more
JEREMY K. NICHOLSON. Section of Biomolecular Medicine, Imperial College London, UK — read more
J. B. BROOKS. Center for Disease Control, Atlanta, USA — read more
N. BELOBORODOVA. Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia
P.D. NICHOLS. Research Scientist, CSIRO, Canberra, Australia
C. W. MOSS. Case Western Reserve University, Cleveland, USA
E.YANZEN. GALAB Laboratories GmbH, Hamburg, Germany
D.B. DRUCKER. Department of Bacteriology and Virology, University of Manchester, Lancashire, UK
C.ASSELINEAU. Institute of Pharmacology and Structural Biology, CNRS-Paul Sabatier University research center, Toulouse, France
M. GOODFELLOW. Newcastle University, Newcastle, United Kingdom
D.E. MINNIKIN. Institute of Microbiology and Infection, University of Birmingham, Edgbaston, Birmingham, UK